Synonyms
LSFM
Digitally scanned Lightsheet Microscopy
Selective Plane Illumination Microscopy
Light-sheet fluorescence microscopy
DSLM
SPIM
Lattice Light-sheet Microscopy
Dual-View inverted SPIM
Spherical aberrations assisted Extended Depth-of-field Lightsheet Microscopy
Bessel Beam Lightsheet Microscopy
single objective Selective Plane Illumination Microscopy
Hardware implementations: multidirectional SPIM
LLSM
Clarity Optimized Lightsheet Microscopy
Multiview Selective Plane Illumination Microscopy
MuViSPIM
inverted SPIM
soSPIM
diSPIM
COLM
SPED
mSPIM
iSPIM

Neuroglancer

Description

Web based viewer developped for google for very big data: 

Neuroglancer is a WebGL-based viewer for volumetric data. It is capable of displaying arbitrary (non axis-aligned) cross-sectional views of volumetric data, as well as 3-D meshes and line-segment based models (skeletons). The segmentation has to be done before loading the dataset, it is not done Inside the viewer.

This is not an official Google product.

It has among other the nice feature of beeing able to generate url for sharing a specific view.

Note that the only supported browser for now are 

  • Chrome >= 51
  • Firefox >= 46

 

Neuroglancer

Tumor Blood Vessels: 3D Tubular Network Analysis

Bioimage Analyst
Developer

In this session, we will implement a simple ImageJ macro to segment and analyze the blood vessel network of a subcutaneous tumor. The analysis is fully performed in 3D, and possible strategies to extract statistics of the network geometry and interactively visualize the results are also discussed and implemented.

Automated workflow for parallel Multiview Reconstruction

Description

Automated workflow for performing multiview reconstruction of large multiview, multichannel, multiillumination time-lapse SPIM data on a high performance computing (HPC) cluster or on a single workstation. 

Spimagine

Description

Spimagine is a python package to interactively visualize and process time lapsed volumetric data as generated with modern light sheet microscopes (hence the Spim part). The package provides a generic 3D+t data viewer and makes use of GPU acceleration via OpenCL. If provides further an image processor interface for the GPU accelerated denoising and deconvolution methods of gputools.

It is only for display (no analysis). The only drawback: it does not handle multichannel time lapse 3D data (only one channel at a time).

has function
Spimagine

Microscope autopilot

Description

AutoPilot is the open source project that hosts the general algorithm for fast and robust assessment of local image quality, an automated computational method for image-based mapping of the three-dimensional light-sheet geometry inside a fluorescently labeled biological specimen, and a general algorithm for data-driven optimization of the system state of light-sheet microscopes capable of multi-color imaging with multiple illumination and detection arms.

has function

Quality metric of 3D SPIM stacks

Description

An ImageJ/Fiji macro which measures quality through two stacks of images assumed to be acquired from two opposite angle of views using gray-level standard deviation.

CSBDeep, a toolbox for Content-aware Image Restoration (CARE) in Knime

Description

Deep learning based restoration, with guidelines for training. See also the Fiji plugin.

CSBDeep, a toolbox for Content-aware Image Restoration (CARE) in Fiji

Description

Deep learning for fluorescence image restoration (denoising, deconvolution). Requires training on your data set but the procedure is described.

CARE

MaMuT

Description

MaMuT is an end user plugin that combines the BigDataViewer and TrackMate to provide an application that allow browsing, annotating and curating annotations for large image data.

Spark Stitcher

Description

Reconstruct big images from overlapping tiled images on a Spark cluster.

The code is based on the Stitching plugin for Fiji https://github.com/fiji/Stitching

SparkStitching