A workflow is a set of components assembled in some specific order to

  1. Measure and estimate some numerical parameters of the biological system or
  2. Visualization

for addressing a biological question. Workflows can be a combination of components from the same or different software packages using several scripts and manual steps.

ZEN Intellesis Trainable Segmentation

Description

Perform Advanced Image Segmentation and Processing across Microscopy Methods
 

Overcome the bottleneck of segmenting your Materials Science images and use ZEISS ZEN Intellesis, a module of the digital imaging software ZEISS ZEN.
Independent of the microscope you used to acquire your image data, the algorithm of ZEN Intellesis will provide you with a model for automated segmentation after training. Reuse the model on the same kind of data and beneft from consistent and repeatable segmentation, not influenced by the operator. 
ZEN Intellesis offers a straightforward, ease-to-use workflow that enables every microscope user to perform advanced segmentation tasks rapidly.

Highlights

  • Simple User Interface for Labelling and Training
  • Integration into ZEN Measurement Framework
  • Support for Multi-dimensional Datasets
  • Use powerful machine learning algorithms for pixel-based classifcation
  • Real Multi-Channel Feature Extraction
  • Engineered Feature Set and Deep Feature Extraction on GPU
  • IP-Function for creating masks an OAD-enabled for advanced automation
  • Powered by ZEN and Python3 using Anaconda Python Distribution
  • Just label objects, train your model and segment your images – there is no need for expert image analysis skills
  • Segment any kind of image data in 2D or 3D. Use data from light, electron, ion or x-ray microscopy, or your mobile phone
  • Speed up your segmentation task by built-in parallelization and GPU (graphics processing unit) acceleration
  • Increase tolerance to low signal-to-noise and artifact-ridden data
  • Seamless integration in ZEN framework and image analysis wizard
  • Data agnostic
  • Compatibility with 2D, 3D and up to 6D datasets
  • Export of multi-channel or labeled images
  • Exchange and sharing of models
  • GPU computing
  • Large data handling
  • Common and well-established machine learning algorithms
  • SW Trial License available

Fit a model for the growth of yeast cells

Description

This notebook uses the rOMERO-gateway and EBImage to process an Image associated to the paper 'Timing of gene expression in a cell-fate decision system'.

The Image "Pos22" is taken from the dataset idr0040-aymoz-singlecell/experimentA/YDA306_AGA1y_PRM1r_Mating. It is a timelapse Image with 42 timepoints separated by 5 minutes. This Image is used to fit a model for the growth of the yeast cells. The notebook does not replicate any of the analysis of the above mentioned paper.

Its purpose is mainly to demonstrate the use of Jupyter, rOMERO-gateway and EBimage.

 

What it does:

  • For each time point of one movie:
    • Read the image for this time point  from the IDR
    • Threshold the images and count the cells using EBimage functions
  • Fit an exponential model to the count of cells against time to get a coefficient of grow (exponential factor)

 

 

 

has function

Cell or particle counting and scoring the percentage of stained objects

Description

This one example workflow from the Cell Profiler(CP)  Examples . CP is commonly used to count cells or other objects as well as percent-positives, by measuring the per-cell staining intensity. This pipeline shows how to do both of these tasks, and demonstrates how various modules may be used to accomplish the same result. 

In a few words, it used the IdentifyPrimaryObject module of CellProfiler to detect nuclei from a channel (e.g DAPI), then again the same module on another channel to detect another probe (e.g some particular histone)  .

Then objects (nuclei) are related to the second object (Histone), to create a parent child-relation ship: where nuclei can have histone has child. Nuclei are then filtered according to the property of having histone (positive) or not having histone (negtiveobject) related to them.  If needed, nuclei can be expanded in order to include touching object rather than object inside only.

The percentage of positive nuclei vs total number of nuclei can then be computed using the CalculateMath Module.

Positivepercentcell

Cell or particle Counting and scoring stained objects using CellProfiler

Description

This is a Jupyter notebook demonstrating the run of a code from IDR data sets by loading a CellProfiler Pipeline 

The example here is applied on real data set, but does not correspond to a biological question. It aims to demonstrate how to create a jupyter notebook to process online plates hosted in the IDR.

It reads the plate images from the IDR.

It loads the CellProfiler Pipeline and replace the reading modules used to read local files from this defaults pipeline by module allowing to read data remotely accessible.

It creates a CSV file and displays it in the notebook.

It makes some plot with Matplotlib.

 

jupyter

Quantification of outer ring diameters of centriole or PCM proteins of cycling HeLa cells in interphase

Description

This workflow can be ran with data from 3D-SIM showing the centrosomes in order to compare the distribution of diameters of rings (or toroids) of different proteins from the centrioles or the peri centriolar material. It aims to reproduce the results of the Nature Cell Biology Paper Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material  from the same data set but with a different analysis method.

It is slightly different from the methods described in the paper itself, where the method was to work on a maximum intensity projection of a 3D-SIM stack, and then to fit circle to the centrioles to estimate the diameters of the toroids.

In this workflow, the images are read from the IDR , then process by thresholding (Maximum entropy auto thresholding with Image J), and processed by Analyze Particles  with different measurement sets, including the bouding box. Then the analysis of diameters and the statistical test are performed using R. All the code and data sets are available, and in the case of this paper have shown a layered organisation of the proteins.

Combined view from Figure 1 Lawo et al.

HyphaTracker

Description
HyphaTrackerWorkflow
HyphaTracker Workflow

HyphaTracker propose a workflow for time-resolved analysis of conidia germination. Each part of this workflow can also be used independnatly , as a toolbox. It has been tested on bright-field microscopic images of conidial germination. Its purpose is mainly to identify the germlings and to remove crossing hyphae, and measure the dynamics of their growth.

hyphatracker

MAARS

Description

automated open-source image acquisition and on-the-fly analysis pipeline (initially developped for analysis of mitotic defects in fission yeast)

maars workflow from publication

 

maars

bleb dynamics

Description

The purpose of the workflow is ....

First you need

need a thumbnail

FlyLimbTracker

Description

  FlyLimbTracker is  a method that uses active contours to semi-automatically track body and leg segments from video image sequences of unmarked, freely behaving Drosophila flies. This approach can be used to measure leg segment motions during a variety of locomotor and grooming behaviors.

For now the plugin have to be downlaoded directly from the EPFL website (see link), not from the search bar as usual in ICY.

 

Drosophila track legs