Automated

The Sholl technique is used to describe neuronal arbors. This plugin can perform Sholl directly on 2D and 3D grayscale images of isolated neurons. Its internal algorithm to collect data is based upon how Sholl analysis is done by hand — it creates a series of concentric shells (circles or spheres) around the focus of a neuronal arbor, and counts how many times connected voxels defining the arbor intersect the sampling shells. The major advantages of this plugin over other implementations are:

• When analyzing images directly, it does not require previous tracing of the arbor (although it can also analyze traced arbors)
• It combines curve fitting with several methods to automatically retrieve quantitative descriptors from sampled data, which allows direct statistical comparisons between arbors
• It allows continuous and repeated sampling around user-defined foci
• It allows batch processing

Bio-Formats is a standalone Java library for reading and writing life sciences image file formats. It is capable of parsing both pixels and metadata for a large number of formats, as well as writing to several formats. The primary goal of Bio-Formats is to facilitate the exchange of microscopy data between different software packages and organizations. It achieves this by converting proprietary microscopy data into an open standard called the OME Data Model, particularly into the OME-TIFF file format. ### Command Line Tools Bioformats could also be used as stand alone application from command line. See [Bioformats command line tools introduction.](http://www.openmicroscopy.org/site/support/bio-formats5/users/comlineto…)

Processing is a flexible software sketchbook and a language for learning how to code within the context of the visual arts. Since 2001, Processing has promoted software literacy within the visual arts and visual literacy within technology.

This is an icy package that encapsulates tools to design and implement parametric active contours. The package provides fast 2D and 3D filters for image preprocessing, and a framework to create and evolve snakes defined by a set of control points.

This plugin stabilizes jittery image stacks using the Lucas-Kanade algorithm. It supports both grayscale and color images.

See also this tweet

This plugin generates theoretical PSFs, assuming they arise only from diffraction.

This plugins allows debleaching of time sequences of fluorescence images.

Bundled with Fiji. "Do all" is the great feature...

This software implements an active contour (a.k.a. snake) segmentation method using exponential splines as basis functions to represent the outline of the shape. While the snake is versatile enough to provide a good approximation of any closed curve in the plane, its most important feature is that it perfectly reproduces circular and elliptical shapes. These features are very appropriate to delineate cross sections of cylindrical-like conduits and to outline blob-like objects.

This plugin shows the color distribution within a 3D-color-space. Extensive documentation is available atwww.f4.fhtw-berlin.de/~barthel/ImageJ/ColorInspector/help.htm.

Color Inspector 3D is also available as a stand-alone program that uses ImageJ as a library. To run it, download ColorInspector3D.jar and double click on it. On Windows, Java 5.0 or later must be installed

Choose the best auto thresholding technique for your data. 

Diagnose bugs in Icy

View a single channel of a 2D image as a 3D elevation map (X,Y,Intensity).