Estimate the positions and spacing between sections (or at local points) of three dimensional image data. This method may be applied to any imaging modality that acquires 3-dimensional data as a stack of 2-dimensional sections. We provide plugins for both Fiji and TrakEM2.
An ImageJ macro for correcting frame drift occurred during image acquisition.
It often happens that you have an image sequence that shows problematic drifting of image frame and at the same time you have some landmarks that could be used for correcting the drift. This ImageJ macro allows you to Manually track the landmark using ImageJ Manual Tracking Plugin. Using the coordinates recorded in the Result window, each frame is shifted back so that the landmark stays in a single place.
Three different methods for correcting fluorescence bleaching.
1. Simple (framewise ratio based)
2. Exponential (curve fitting with exponential decay model)
3. Histogram matching (register histogram shape. with 16 bit, it takes long time... it should be improved).