Channel shift correction



Acquiarium is open source software (GPL) for carrying out the common pipeline of many spatial cell studies using fluorescence microscopy. It addresses image capture, raw image correction, image segmentation, quantification of segmented objects and their spatial arrangement, volume rendering, and statistical evaluation.

It is focused on quantification of spatial properties of many objects and their mutual spatial relations in a collection of many 3D images. It can be used for analysis of a collection of 2D images or time lapse series of 2D or 3D images as well. It has a modular design and is extensible via plug-ins. It is a stand-alone, easy to install application written in C++ language. The GUI is written using cross-platform wxWidgets library.

Acquiarium functionalities diagram

Detection of Molecules - DoM


A collection of components for super resolution image data:

  • Detect Molecules
  • Reconstruct Image
  • Results table
  • Drift correction
  • Chromatic correction

Multiview Reconstruction


The Multiview Reconstruction software package enables users to register, fuse, deconvolve and view multiview microscopy images. The software is designed for lightsheet fluorescence microscopy (LSFM), but is applicable to any form of three or higher dimensional imaging modalities like confocal timeseries or multicolor stacks. 

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ImageJ Align RGB planes


From the plugin inline help:

" Align RGB planes v1.7 by G.Landini Changes the alignment of the RGB planes independently.

Red Green and Blue checkboxes switch ON and OFF the planes and undo the alignment since last plane change. Note that when switching planes, the portion of the previously edited plane left outside the image frame is lost. Rotation, Width and Height changes are interpolated (so there is some loss of sharpness) and do not retain the image portions outside the image frame. You can use the Resize2Rotate macro to avoid losing any image data.

The RotateWidth and Height sliders set integer values, but fractional values can also be typed in the entry boxes. Just make sure you press [RETURN] after the number is typed.

The Revert button works only with single images, not stacks.

Note: When using stacks, 2 buttons [< Prev] and [Next >] are added to the panel. Do not use the slide bar in the stack window, but use those buttons instead."

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