Align aligns images relative to each other, for example, to correct
shifts in the optical path of a microscope in each channel of a
multi-channel set of images.
For two or more input images, this module determines the optimal
alignment among them. Aligning images is useful to obtain proper
measurements of the intensities in one channel based on objects
identified in another channel, for example. Alignment is often needed
when the microscope is not perfectly calibrated. It can also be useful
to align images in a time-lapse series of images. The module stores the
amount of shift between images as a measurement, which can be useful for
quality control purposes.
Note that the second image (and others following) is always aligned with
respect to the first image. That is, the X/Y offsets indicate how much
the second image needs to be shifted by to match the first.
This module does not perform warping or rotation, it simply shifts images
in X and Y. For more complex registration tasks, you might preprocess
images using a plugin for that purpose in FIJI/ImageJ.
Measurements made by this module
- Xshift, Yshift: The pixel shift in X and Y of the aligned image
with respect to the original image.
- Lewis JP. (1995) “Fast normalized cross-correlation.” Vision