Synonyms
Tracking

Filopodyan

Description

Fiji plugin for detecting, tracking and quantifying filopodia

EB1 tracking with Matlab

Bioimage Analyst
Developer

This module follow EB1 tracking with IJ. In this session, we will visualize the tracking results and also cover typical analysis protocols for the quantitative analysis of movement. Two dynamic numerical features could be extracted from tracking results: speed and direction. Estimation of movement speed from multiple trajectories is a popular indicator of movement, and we will quickly go over the method for estimating the average speed of EB1 movement along microtubule. Movement direction is another quantitative feature, but is rarely explored.

EB1 tracking with IJ

Bioimage Analyst
Developer

We take an example image data of microtubule binding protein EB1, and will study how to automatically track those signals and how to analyze the tracking results. We use ImageJ for measuring the temporal changes in signal positions, and will feed the tracking results for analyzing their dynamics using Matlab in the following session EB1 tracking with Matlab.

Nuclei Tracking (ImageJ)

has function
need a thumbnail

Manual tracking with TrackMate

Description

Manual tracking using Trackmate plugin (comes with FIji, so no installation required if you are using Fiji). 

has function

HyphaTracker

Description
HyphaTrackerWorkflow
HyphaTracker Workflow

HyphaTracker propose a workflow for time-resolved analysis of conidia germination. Each part of this workflow can also be used independnatly , as a toolbox. It has been tested on bright-field microscopic images of conidial germination. Its purpose is mainly to identify the germlings and to remove crossing hyphae, and measure the dynamics of their growth.

hyphatracker

MaMuT

Description

MaMuT is an end user plugin that combines the BigDataViewer and TrackMate to provide an application that allow browsing, annotating and curating annotations for large image data.

kymograph generation

Description

Kymograph generation under ImageJ:

one simple solution, plot a line (ROI line) on the first frame, where you want to generate the kymograph.

Use

Image  / Stacks  / Reslice

It will generate a new image were Y dimension is the time, and X the position on the line you have drawn.

need a thumbnail

FlyLimbTracker

Description

  FlyLimbTracker is  a method that uses active contours to semi-automatically track body and leg segments from video image sequences of unmarked, freely behaving Drosophila flies. This approach can be used to measure leg segment motions during a variety of locomotor and grooming behaviors.

For now the plugin have to be downlaoded directly from the EPFL website (see link), not from the search bar as usual in ICY.

 

Drosophila track legs