Description
Microtubule end tracking in live cell fluorescent images of Drosophila oocyte involves overcoming the following challenges, which can be tackled by a series of preprocessing steps and tracking described in Parton et al (2011)
* **illumination flicker & photobleaching**: suppress by normalising intensities, e.g. using Image->Adjust->Bleach Correction in Fiji/ImageJ
* **uneven illumination**: Fourier bandpass filtering (e.g. Process->FFT->Bandpass Filter) preserves features within a selected size range
* **high background / poor contrast**: foreground filter, e.g. Temporal Median filter
* **tracking**: e.g. TrackMate in Fiji/ImageJ (segmentation using DoG detector)