Confocal fluorescence microscopy

Confocal fluorescence microscopy

Synonyms
Laser scanning microscopy
Nipkow disk microscopy
Spinning Disk confocal microscopy
LSM
SDCM

Performing a Fluorescence Recovery after Photobleaching (FRAP) experiment

Workflow
Description

The article describes how a FRAP experiment can be conducted and subsequently analyzed. This includes steps in ImageJ and subsequent normalization of the intensity data.

This is a qualitative analysis, and curve fitting is done using Excel. 

Requires "Template matching and Slice alignment plugin"

has topic
Confocal fluorescence microscopy

2D and 3D mitochondrial shape analysis and network properties

Workflow
Description

The original paper describes a method to analyze mitochondrial morphology in 2D and 3D.

has topic
Confocal fluorescence microscopy
has function
Analysis
Image segmentation

Huygens

Collection
Description

The Huygens Software Suite consists of different image processing packages with functionalities that include deconvolution, interactive analysis, and volume visualization of 2D-3D multi-channel and time series images from fluorescence microscopes such as widefield, confocal, multi-photon, spinning disk, Array Detector, STED, and Light Sheet

has topic
Light-sheet microscopy
Widefield microscopy
Fluorescence microscopy
Confocal fluorescence microscopy
Super-resolution microscopy
has function
Colocalisation analysis
Object-based colocalisation
Single molecule localisation
Object tracking
Image deconvolution
Chromatic aberration correction
Image visualisation
Volume rendering
Subscribe to Confocal fluorescence microscopy