2D

NODeJ is an ImageJ plugin for 3D segmentation of nuclear objects.

"The three-dimensional nuclear arrangement of chromatin impacts many cellular processes operating at the DNA level in animal and plant systems. Chromatin organization is a dynamic process that can be affected by biotic and abiotic stresses. Three-dimensional imaging technology allows to follow these dynamic changes, but only a few semi-automated processing methods currently exist for quantitative analysis of the 3D chromatin organization. We present an automated method, Nuclear Object DetectionJ (NODeJ), developed as an imageJ plugin. This program segments and analyzes high intensity domains in nuclei from 3D images. NODeJ performs a Laplacian convolution on the mask of a nucleus to enhance the contrast of intra-nuclear objects and allow their detection. We reanalyzed public datasets and determined that NODeJ is able to accurately identify heterochromatin domains from a diverse set of Arabidopsis thaliana nuclei stained with DAPI or Hoechst. NODeJ is also able to detect signals in nuclei from DNA FISH experiments, allowing for the analysis of specific targets of interest. NODeJ allows for efficient automated analysis of subnuclear structures by avoiding the semi-automated steps, resulting in reduced processing time and analytical bias. NODeJ is written in Java and provided as an ImageJ plugin with a command line option to perform more high-throughput analyses. NODeJ can be downloaded from https://gitlab.com/axpoulet/image2danalysis/-/releases with source code, documentation and further information avaliable at https://gitlab.com/axpoulet/image2danalysis . The images used in this study are publicly available at https://www.brookes.ac.uk/indepth/images/ and https://doi-org.osaka-u.idm.oclc.org/10.15454/1HSOIE ."

An imageJ/Fiji plugin that measures and classifies neurites from a very large number of neurons.

BraiAn is an open-source suite of tools designed to simplify signal quantification, analysis and visualization of large datasets typically obtained in whole-brain imaging experiments, following registration to an atlas. 

The package consists of two separate modules.

1. BrainAnDetect: A QuPath extension for multi-channel cell segmentation across large and variable datasets. It leverages QuPath's built in algorithms for cell detection, and features additional options for refining signal quantification, including machine-learning-based object classification, region-specific cell segmentation, multiple marker co-expression analysis, and an interface for selective exclusion of damaged tissue portions.
2. BraiAnalyse: A modular Python library for the easy navigation, visualization, and analysis of whole-brain quantification outputs.

A generalist framework for multi-dimensional automatic spot detection and quantification.

SpotMAX is designed to accomplish two tasks:

1. Detecting and quantifying globular-like structures (a.k.a. "spots")
2. Segmenting and quantifying fluorescently labelled structures

It supports 2D, 3D, 4D, and 5D data, i.e., z-stacks, timelapse, and multiple fluorescence channels (and combinations thereof).

This workflow is the integration of YOLO (You Only Look Once) machine learning models, image pre-processing scripts and labeling tools within the Galaxy platform. Galaxy is an open, web-based platform used primarily for data analysis in computational biology, but it also has applications in image processing and other fields. 

How the Galaxy YOLO image segmentation tool works

The combination of Galaxy and YOLO allows researchers to perform object detection and image analysis without requiring extensive programming knowledge. Here's how it generally works: 

• Web-based interface: Galaxy provides a graphical, user-friendly interface to access powerful analysis tools. Users can simply upload their image data, select the YOLO tool, and run the analysis.
• YOLO model execution: The Galaxy tool executes a pre-trained YOLO model, often from the Ultralytics framework, on the input images. These models can perform tasks like object detection (drawing bounding boxes) or instance segmentation (creating pixel-level masks).
• Training and prediction: Some tools allow for both model training and prediction. Users can train a custom YOLO model on their own labeled datasets to detect specific objects of interest. For example, bioimage analysis may involve detecting cells or other structures.
• Other integrations: Other machine-learning tools can be integrated with YOLO in Galaxy. For instance, the AnyLabeling tool supports YOLO for semi-automated and active learning-based data annotation.