Digital histology

MembraneQuant performs automatic evaluation on IHC membrane stainings (HER2, EGFR etc).

Using color deconvolution, MembraneQuant detects cell membrane and measures staining intensity on the chromogen channel. This way it is possible to calibrate the software to the actual stain protocol in the pathology lab. The algorithm categorizes the detected membrane to weak positive, medium positive and strong positive classes.

This software has an IVD certification for HER2 quantification.

When opening the Pannoramic Viewer you see all of your virtual slides in thumbnail view. Selecting one (or up to 10 at a time) the slide gets under the virtual objective of the virtual microscope. Here you can move and change the magnification of the slide quickly and easily using the mouse. Emphasizing 'quickly' is important considering the fact that the size of an average virtual slide can easily be more than 1 GB.

Main characteristics:

• Seamless zooming and moving of the virtual slide
• Bookmarking (annotating) on the spot, i.e. defining the specific part of the sample by drawing; finding and reading of previously made bookmarks
• Easy and precise measurements
• Real-time changing of brightness, contrast and color bias
• Fluorescent slide handling, separate channel view & pseudo-colorization
• Slide uploading and downloading for teleconsultation
• Synchronized viewing (moving and zooming) of multiple slides for comparison purposes
• Publication quality image capture of displayed areas (.JPG, .BMP, .TIFF)
• TIFF, MIRAX slide and Meta-XML export for Carl Zeiss AxioVision™ compatibility
• Scanmap export for rescanning existing digital slides
• Easily expandable functionality via the software modules

NuclearQuant is a QuantCenter module. It is designed for cell nuclei detection and quantification of IHC stained samples. NuclearQuant measures several morphological features besides stain intensity. The cell nuclei classification and the final score are calculated by the intensity score and the proportion score.

DensitoQuant is a simple and fast, yet effective tool for IHC measurements. It measures the density of immunostain on the digital slides by distributing pixels to negative and 3 grades of positive classes by their RGB values. DensitoQuant is especially suitable for quick TMA evaluation. Analyzing a whole digital slide takes only a couple of minutes.