Object detection

Synonyms
Particle detection
Isolated object detection
Description

NuclearQuant is a QuantCenter module. It is designed for cell nuclei detection and quantification of IHC stained samples. NuclearQuant measures several morphological features besides stain intensity. The cell nuclei classification and the final score are calculated by the intensity score and the proportion score.

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NuclearQuant
Description

This module identifies objects that are contained within in a grid pattern, allowing you to measure the objects using Measure modules. It requires you to have defined a grid earlier in the pipeline, using the DefineGrid module. For several of the automatic options, you will need to enter the names of previously identified objects. Typically, this module is used to refine locations and/or shapes of objects of interest that you roughly identified in a previous Identify module. Within this module, objects are re-numbered according to the grid definitions rather than their original numbering from the earlier Identify module. If placing the objects within the grid is impossible for some reason (the grid compartments are too close together to fit the proper sized circles, for example) the grid will fail and processing will be canceled unless you choose to re-use a grid from a previous successful image cycle.

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