Human cytoplasm-nucleus translocation assay using CellProfiler

Type
Author
Cellprofiler team
Requires
Execution Platform
Implementation Type
Supported image dimension
Interaction Level
License/Openness
Description

In this human cytoplasm-nucleus translocation assay, learn how to load a previously calculated illumination correction function for two separate channels, measure protein content in the nucleus and cytoplasm, and calculate the ratio as a measure of translocation. This is a clumpy cell type, so studying the settings in primary object identification may be helpful for users interested in the more advanced options that module offers. More about these images can be found at the BBBC.

Additional keywords
Entry Curator
migration