This plugin computes for each image element (pixel/voxel) the eigenvalues of the Hessian, which can be used for example to discriminate locally between plate-like, line-like, and blob-like image structures
This workflow detects spots in a 2D image by filtering the image by Laplacian of Gaussian (user defined radius), thresholding (user defined threshold) and finding local intensity maxima in mask distance map (Dmap).
Starting from image stacks, the nuclear boundary as well as nuclear bodies are segmented. As output, NucleusJ automatically measures 15 parameters quantifying shape and size of nuclei as well as intra-nuclear objects and the positioning of the objects within the nuclear volume.
This plugin ships automated methods for extracting trajectories of multiples objects in a sequence of 2D or 3D images. Up to version 2 it was known as the ‘Probabilistic particle tracker’ plugin.