Semi-automated

This ImageJ plugin segments the image in classes by thresholding. It uses the same algorithm found in Otsu Thresholding, but was adapted to output more than 2 classes out of the process.

Identify objects (as nuclei) within an image without needing the assistance of another cellular feature (as cell). 

When opening the Pannoramic Viewer you see all of your virtual slides in thumbnail view. Selecting one (or up to 10 at a time) the slide gets under the virtual objective of the virtual microscope. Here you can move and change the magnification of the slide quickly and easily using the mouse. Emphasizing 'quickly' is important considering the fact that the size of an average virtual slide can easily be more than 1 GB.

Main characteristics:

• Seamless zooming and moving of the virtual slide
• Bookmarking (annotating) on the spot, i.e. defining the specific part of the sample by drawing; finding and reading of previously made bookmarks
• Easy and precise measurements
• Real-time changing of brightness, contrast and color bias
• Fluorescent slide handling, separate channel view & pseudo-colorization
• Slide uploading and downloading for teleconsultation
• Synchronized viewing (moving and zooming) of multiple slides for comparison purposes
• Publication quality image capture of displayed areas (.JPG, .BMP, .TIFF)
• TIFF, MIRAX slide and Meta-XML export for Carl Zeiss AxioVision™ compatibility
• Scanmap export for rescanning existing digital slides
• Easily expandable functionality via the software modules

Analyze 12.0 is a set of image processing, visualization and analysis tools that provides users access to a breadth of essential post-processing functions critical for innovative research. Analyze 12.0 provides users with all the key display, editing, processing, registration, and measurement tools needed to realize their research goals in a single multifaceted software package. Analyze 12.0 is open-ended, allowing for the application of its tools to novel and emerging research.