Windows

Description

The FindFoci plugins allow the identification of peak intensity regions within 2D and 3D images. The algorithm is highly configurable and parameters can be optimised using reference images and then applied to multiple images using the batch mode. Details of the benefits of training an algorithm on multiple images can be found in the FindFoci paper: 2591

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Description

Quote: *A GUI-based program which manually detects spots and places them into previously detected meshes. Currently the program runs from MATLAB only. *

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Description

Quote: *SpotFinderZ (from now on simply SpotFinder) detects round, usually diffraction-limited spots inside bacterial cells outlined with MicrobeTracker and places them into the meshes structure produced by MicrobeTracker. The program is written in MATLAB and saves the data in the MicrobeTracker format by appending additional fields.*

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Description

The Fourier transform of an image produces a representation in frequency space: i.e. separated according to spatial frequency (effectively scale). The 2D amplitude map of the different spatial frequencies is symmetrical, and is commonly displayed with low spatial frequencies (large features) in the centre, highest spatial frequencies (small features) at the edges. Fourier filtering involves suppressing or enhancing features in the Fourier domain before carrying out an inverse Fourier transform to obtain a filtered real-space image. ImageJ's _Process > FFT > Bandpass Filter_ implements two common Fourier-filtering functions: 1. filtering for specific sizes of feature in an image by selecting minimum and maximum feature sizes (selecting a radial band of frequencies in Fourier space); 2. filtering out repetitive horizontal or vertical stripes by cutting out a zero-frequency stripe in the orthogonal direction in frequency space. The example image above shows the effect of filtering for 2 feature size ranges: 0-8 pixels, and 8-256 pixels; where the former appears "flattened" or washed-out, and the latter very blurred. The small images displayed to the lower-right of each filtered image correspond to the mask applied to the Fourier transform. Such filtering can be useful prior to global thresholding, for noise suppression, etc.

ImageJ bandpass screenshot
Description

Implementation of some image correlation spectroscopy tools

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