Image Analysis of Biological Data using CellProfiler
After the session you will be able to built your own CellProfiler pipeline, including:
- Image data import
- Object segmentation (e.g. detect nuclei in an image) using the modules "IdentifyPrimaryObjects" and "IdentifySecondaryObjects"
- Object feature measurements (e.g. measure size, shape and intensity of cells)
- Measurements export to a spreadsheet
- Creating and saving quality control images
- 83 views
Tool(s) used for training
Date
Prerequisites
To be able to follow the course you need to know basic image analysis:
- concept of “images are a matrix of numbers”
- handling of multi-channel images
- bit-depth
- segmentation by applying an global threshold
- “analyze particles” (connected component analysis)
Format
License
cc-by
Expected duration
1hours
