R

Description

The Image Data Explorer is a Shiny app that allows the interactive visualization of images and ROIs associated with data points shown in a scatter plot. It is useful for exploring the relationships between images/ROIs and associated data represented in tabular format.

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Description

The goal of mamut2r is to imports data coming from .xml files generated with the Fiji MaMuT plugin for lineage and tracking of biological objects. {mamut2r} also allows to create lineage plots.

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Description

CRImage a package to classify cells and calculate tumour cellularity

CRImage provides functionality to process and analyze images, in particular to classify cells in biological images. Furthermore, in the context of tumor images, it provides functionality to calculate tumour cellularity.

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Description

shinyHTM is an open source, web-based tool for data exploration, image visualization and normalization of High Throughput Microscopy data. Within shinyHTM the user is guided through a linear workflow which follows the following best practices:

  • Inspect the numerical data through plotting
  • Measurements are linked to raw images
  • Perform quality control to exclude images with aberrations or where image analysis failed
  • Perform a reproducible data analysis
  • Normalize data and report statistical significance

Image visualization relies on Fiji/ImageJ, along with its wealth of analytical tools.

shinyHTM can be used to analyze image features obtained with CellProfiler, ImageJ or any other bioimage analysis software. The output of analysis is a publication-ready scoring of the data.

shinyHTM is based on the R shiny package.

shinyHTM
Description

This notebook uses the rOMERO-gateway and EBImage to process an Image associated to the paper 'Timing of gene expression in a cell-fate decision system'.

The Image "Pos22" is taken from the dataset idr0040-aymoz-singlecell/experimentA/YDA306_AGA1y_PRM1r_Mating. It is a timelapse Image with 42 timepoints separated by 5 minutes. This Image is used to fit a model for the growth of the yeast cells. The notebook does not replicate any of the analysis of the above mentioned paper.

Its purpose is mainly to demonstrate the use of Jupyter, rOMERO-gateway and EBimage.

 

What it does:

  • For each time point of one movie:
    • Read the image for this time point  from the IDR
    • Threshold the images and count the cells using EBimage functions
  • Fit an exponential model to the count of cells against time to get a coefficient of grow (exponential factor)

 

 

 

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