Free and open source

mahotas.convolve(f, weights, mode='reflect', cval=0.0, out={new array})

Convolution of f and weights

Convolution is performed in doubles to avoid over/underflow, but the result is then cast to f.dtype. This conversion may result in over/underflow when using small integer types or unsigned types (if the output is negative). Converting to a floating point representation avoids this issue:

c = convolve(f.astype(float), kernel)

ImarisReader is a set of classes for reading the data stored in ims files. ImarisReader can read the primary image data, as well as the data for segmented objects: Cells, Filaments, Spots and Surfaces.

FLIMfit is an OMERO client-side MATLAB application for fitting and visualising time-resolved FLIM data. It supports key data formats including:

• Becker and Hickl .sdt
• LaVision BioTech .msr
• PicoQuant .bin
• OME-TIFF files;

Several options for curve fitting are available. Results can be saved back in OMERO, which also facilitates working collaboratively on the data analysis.