Semi-automated quantification of three stages of phagocytosi using ImageJ

Type
Workflow
Author
Nicholas Ahti Hamilton
Requires
ImageJ
Execution Platform
Linux
Mac
Windows
Programming Language
ImageJ Macros
is compatible with
ImageJ
Supported image dimension
2D
Interaction Level
Semi-automated
License/Openness
Free and open source
License
free
Description

The authors present an ImageJ-based, semi-automated phagocytosis workflow to rapidly quantitate three distinct stages during the early engulfment of opsonized beads.

has function
Cell segmentation
Interactive segmentation
Object classification
has topic
Fluorescence microscopy
Bright field microscopy
has biological terms
Phagocytosis
bead
transfected
Entry Curator
czhang
Post date
04/25/2023 - 17:09
Last modified
05/17/2023 - 16:27
Workflow steps
Step 1: segment beads (from bright field image)
ImageJ Threshold
Image thresholding
Step 2: filter segmented objects by size (from bright field image)
Analyze Particles
Object detection
Step3: segment beads (from fluorescence image)
ImageJ Threshold
Image thresholding
Step 4: classify according to overlap percentage between both segmention objects
ImageJ Macro
Object-based colocalisation
Download Page
ImageJ macros
Reference Publication Link
High-throughput quantification of early stages of phagocytosis
Image-Based Analysis of Phagocytosis: Measuring Engulfment and Internalization
Usage Example Link
User manual