Imaging

Description

Measures wound-healing assay videos, 

 For each video, the velocity and the order parameter are analyzed in time and space to extract quantitative parameters characterizing the cell motility phenotype. The different conditions (videos) can then be classified according to these parameters.

AveMAP
Description

The invention comprises a software tool, NeuronMetrics, which functions as a set of modules that run in the open-source program ImageJ. NeuronMetrics features a novel method for estimating neural “branch number” (a measure of the axonal complexity) from two-dimensional images. In addition, the tool features a novel method for modeling neural structure in large “gaps” that result from image artifacts.

 

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Description

Computes image gradient

 

Based on the algorithm below. 

Splines: A Perfect Fit for Signal and Image Processing
M. Unser
IEEE Signal Processing Magazine, vol. 16, no. 6, pp. 22-38, November 1999.
 DOI: 10.1109/79.799930
 http://ieeexplore.ieee.org/document/799930/

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Description

GelBandFitter is a user-friendly software specific for analysis of protein gels and estimation of relative protein content. Using non-linear regression methods to fit mathematical functions to densitometry profiles, it is able to estimate content from protein bands that partially overlap. The software is available either as Matlab code (Optimization toolbox required) or a Windows executable. Reference: Mitov, M. I., Greaser, M. L., & Campbell, K. S. (2009). GelBandFitter – A computer program for analysis of closely spaced electrophoretic and immunoblotted bands. Electrophoresis, 30(5), 848–851. http://doi.org/10.1002/elps.200800583

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GelBandFitter screenshot
Description

Estimate the frequency of hepatitis C virus infected cells based on the intensity of viral antigen associated immunofluorescence. 

The core is an ImageJ Macro, so it's easy to modify for one's own needs (Link to the code). 

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