Quote: "The GDSC ImageJ plugins are a collection of analysis programs for microscopy images including colocalisation analysis and peak finding (FindFoci)." Many types of analysis besides simply finding foci detection (spot detection) is bundled in this plugin. One prominent function is "FindFoci Optimizer". This allows feeding images with spot annotation by the user (multi-point selection tool) and scans through various parameter combinations to find the best parameter set that gives the results similar to the annotation. This is almost like machine learning... but with well-established parameter types that allows you to fully understand what is going on.