2D spots counting using KNIME
Supported image dimension
These two KNIME workflow solutions are similar: first one detects nuclei and spots inside the nuclei without taking care of surrounding regions, i.e. mitochondria. The second one provides the full solution including spots in mitochondria.
see section 2.4 for KNIME workflow. Section 2.3 is also available, using Fiji.
Sample image: hela-cells.tif (674k x 3)
has biological terms