Type
Author
Benjamin Gyori
Gireedhar Venkatachalam
Requires
Execution Platform
Programming Language
Supported image dimension
Interaction Level
License/Openness
Description

This workflow will batch process a directory of images: - comets should be horizontally oriented, tails to the right. Additional preprocessing is required if the gel does not match with this orientation (Rotate images, Using ImageJ/Transform Image or TransformJ plugin for example). Then using the plugin:

  1. Uneven background correction
  2. Automatic detection of comet shapes with outliers detection
  3. Automatic detection of theĀ heads of cometsĀ (brightest region or profile analysis)
  4. Statistical values of tails, heads and Olive moments.
  • Manual correction is available.
  • Does live analysis with Micro-Manager
has biological terms
Entry Curator