Component

A utility macro for the specified use of BioFormats plugin. Takes a folder of proprietary images formats (Zeiss zvi, lsm, czi or Nikon nd2) and extracts them to .tif images

The extracted images are located in a folder defined in the menu. Other options: reset spatial scales, reads ROIs, split channels, add stage position in the name.

note: The old name of the macro was "ZVI Extractor" and the data format was limited to ZVI, but the upgraded version includes more formats.

Requires Bio-Formats plugin

This protocol computes the optical flow of a 2D+T sequence. The results are displayed with flow arrows painted on top of the original sequence, and also with two additional sequences for the norm of the flow and a color-coded presentation of the flow following the Middlebury convention.

This macro is similar to the LIF_Extractor, but it will output Z-projection of each Z-stack. You can choose the type of projection in addition to the other options. Requires Bio-Formats plugin

This macro extracts .lei and .lif multichannel Z-stacks into multiple .tif stacks, splitting the channels into different stacks. Several options are possible such as background substraction, various filters, or optional reset of spatial scale. Requires Bio-Formats plugin

This workflow classifies, or segments, the pixels of an image given user annotations. It is especially suited if the objects of interests are visually (brightness, color, texture) distinct from their surrounding. Users can iteratively select pixel features and provide pixel annotations through a live visualization of selected feature values and current prediction responses. Upon users' satisfaction, the workflow then predicts the remaining unprocessed image(s) regions or new images (as batch processing). Users can export (as images of various formats): selected features, annotations, predicted classification probability, simple segmentation, etc. This workflow is often served as one of the first step options for other workflows offered by ilastik, such as object classification, automatic tracking.