Analysis of Microtubule Orientation: Tracking with ImageJ, Directionality Analysis with Matlab

Submitted by Perrine on Mon, 04/08/2019 - 14:02

We take an example image data of microtubule binding protein EB1, and will study how to automatically track those signals and how to analyze the tracking results. We use ImageJ for measuring the temporal changes in signal positions, and will feed the tracking results for analyzing their dynamics using Matlab in the following session.

Visualization of 3D images with Matlab

Submitted by Perrine on Mon, 04/08/2019 - 13:58

In this session we will use a 3D multichannel reconstruction of zebrafish larva to explore the visualization capabilities of Matlab. We will start from extracting and inspecting single slices and will continue with combining multiple channels, finally generating a surface rendering for visual colocalization analysis.During the process we will review methods for manipulating multidimensional arrays, including resizing, reshaping and conditional selection.