Introduction to ImageJ macros: Quantify the enrichment of NPC proteins at the nuclear envelope, relative to its cytoplasmic localisation
ImageJ, for those with GUI knowledge but without scripting knowledge
Workflow
A workflow is a set of components assembled in some specific order to
- Measure and estimate some numerical parameters of the biological system or
- Visualization
for addressing a biological question. Workflows can be a combination of components from the same or different software packages using several scripts and manual steps.
Working with objects: measurements in 2D and 3D
ImageJ, GUI training for beginners, Count and Measure 2D nuclei,Count number of foci on 3D image , compare 2D, 2.5D, 3D results (simple workflows)
Analysis of Microtubule Orientation: Tracking with ImageJ, Directionality Analysis with Matlab
We take an example image data of microtubule binding protein EB1, and will study how to automatically track those signals and how to analyze the tracking results. We use ImageJ for measuring the temporal changes in signal positions, and will feed the tracking results for analyzing their dynamics using Matlab in the following session.
From workflows to simple batch macros: Quantify intensity at the nuclear envelope.
ImageJ, for those with GUI knowledge but without scripting knowledge.
From workflows to simple batch macros: Measure Phalloidin in the nucleus area.
ImageJ, for those with GUI knowledge but without scripting knowledge