LIVECell is a manually annotated and expert-validated dataset of 2D phase contrast images, consisting of over 1.6 million cells from a diverse set of cell morphologies and culture densities. It is also associated with some trained models. All are published under CC BY-NC 4.0 license.
The full datset is described in Edlund, C., Jackson, T.R., Khalid, N. et al. LIVECell—A large-scale dataset for label-free live cell segmentation. Nat Methods 18, 1038–1045 (2021). https://doi-org.insb.bib.cnrs.fr/10.1038/s41592-021-01249-6.
Annotations are in JSON format and follow the COCO - Common Objects in Context (cocodataset.org) format.
Several wells for each cell type were seeded in 96-well plates (Corning) and imaged over the course of 3–5 d, every 4 h using an Incucyte S3 Live-Cell Analysis system (Sartorius) equipped with its standard CMOS camera (Basler acA1920-155um). The Incucyte HD phase-contrast imaging algorithm allows visualization of phase delays produced by cells without the phase annulus or phase ring found in conventional Zernike phase images. Because of this, LIVECell phase-contrast images are characterized by less pronounced halo artifacts and more high-frequency content than other phase-contrast modalities. Phase-contrast images were acquired using a ×10 objective from two positions in each well adding up to a total of 1,310 images (1,408 × 1,040 pixels corresponding to 1.75 × 1.29 mm2) that were each cropped into four equally sized images (704 × 520 pixels corresponding to 0.875 × 0.645 mm2) that were then annotated.