The ImageJ pligin, called PixFRET, allows a simple and rapid determination of channel bleed-through parameters and the display of normalized FRET images. see 2521


Input data type: 

Stacks with 2 channels (controls for bleed-through calculation) or 3 channels (FRET calculation)

Output data type: 

One Image with FRET values and One image with normalized FRET values