fluorescence lifetime imaging microscopy


Software for analysis, visualization, simulation, and acquisition  of data from spectroscopy and fluorescence microscopy.

  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Imaging (FLIM) and Phasor plots
  • Förster Resonance Energy Transfer (FRET)
  • Generalized Polarization (GP) and Spectral Phasors
  • Number and Brightness (N&B)
  • Photon Counting Histogram (PCH)
  • Raster and Spatio-temporal Image Correlation Spectroscopy (RICS and STICS)
  • Single Particle and Modulation Tracking (SPT, MT)
  • Image Mean Square Displacement (iMSD)
  • Pair correlation function (pCF)
has function

A complete package for fluorescence lifetime analysis implemented as an R package with sample data.

An exponential curve fitting library used for Fluorescence Lifetime Imaging (FLIM) and Spectral Lifetime Imaging (SLIM), available as: * [Source code as C/C++ and Java library](https://github.com/slim-curve) * [ImageJ plugin](http://fiji.sc/SLIM_Curve) * [Standalone Open-Source Windows application](https://www.assembla.com/spaces/ATD_TRI/wiki/Home) Publications: * [Barber, P.R., Proc. SPIE, 5700, 2005.pdf](http://users.ox.ac.uk/~atdgroup/publications/Barber,%20P.R.,%20Proc.%20SPIE,%205700,2005.pdf) * [Barber, P.R., J. R. Soc. Interface, 6, 2009.pdf](http://users.ox.ac.uk/%7Eatdgroup/publications/Barber,%20P.R.,%20J.%20R.%20Soc.%20Interface,%206,%202009.pdf)
FLIMfit is an OMERO client-side MATLAB application for fitting and visualising time-resolved FLIM data. It supports key data formats including: - Becker and Hickl .sdt - LaVision BioTech .msr - PicoQuant .bin - OME-TIFF files; Several options for curve fitting are available. Results can be saved back in OMERO, which also facilitates working collaboratively on the data analysis.