fluorescence lifetime imaging microscopy


Software for analysis, visualization, simulation, and acquisition  of data from spectroscopy and fluorescence microscopy.

  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Imaging (FLIM) and Phasor plots
  • Förster Resonance Energy Transfer (FRET)
  • Generalized Polarization (GP) and Spectral Phasors
  • Number and Brightness (N&B)
  • Photon Counting Histogram (PCH)
  • Raster and Spatio-temporal Image Correlation Spectroscopy (RICS and STICS)
  • Single Particle and Modulation Tracking (SPT, MT)
  • Image Mean Square Displacement (iMSD)
  • Pair correlation function (pCF)
has function

A complete package for fluorescence lifetime analysis implemented as an R package with sample data.


An exponential curve fitting library used for Fluorescence Lifetime Imaging (FLIM) and Spectral Lifetime Imaging (SLIM), available as:



FLIMfit is an OMERO client-side MATLAB application for fitting and visualising time-resolved FLIM data. It supports key data formats including:

  • Becker and Hickl .sdt
  • LaVision BioTech .msr
  • PicoQuant .bin
  • OME-TIFF files;

Several options for curve fitting are available. Results can be saved back in OMERO, which also facilitates working collaboratively on the data analysis.