fluorescence lifetime imaging microscopy

Software for analysis, visualization, simulation, and acquisition  of data from spectroscopy and fluorescence microscopy.

• Fluorescence Correlation Spectroscopy (FCS)
• Fluorescence Lifetime Imaging (FLIM) and Phasor plots
• Förster Resonance Energy Transfer (FRET)
• Generalized Polarization (GP) and Spectral Phasors
• Number and Brightness (N&B)
• Photon Counting Histogram (PCH)
• Raster and Spatio-temporal Image Correlation Spectroscopy (RICS and STICS)
• Single Particle and Modulation Tracking (SPT, MT)
• Image Mean Square Displacement (iMSD)
• Pair correlation function (pCF)

A complete package for fluorescence lifetime analysis implemented as an R package with sample data.

An exponential curve fitting library used for Fluorescence Lifetime Imaging (FLIM) and Spectral Lifetime Imaging (SLIM), available as:

• Source code as C/C++ and Java library

• ImageJ plugin

• Standalone Open-Source Windows application

Publications:

• Barber, P.R., Proc. SPIE, 5700, 2005.pdf
• Barber, P.R., J. R. Soc. Interface, 6, 2009.pdf

FLIMfit is an OMERO client-side MATLAB application for fitting and visualising time-resolved FLIM data. It supports key data formats including:

• Becker and Hickl .sdt
• LaVision BioTech .msr
• PicoQuant .bin
• OME-TIFF files;

Several options for curve fitting are available. Results can be saved back in OMERO, which also facilitates working collaboratively on the data analysis.