2D

Crop crops or masks an image.

This module crops images into a rectangle, ellipse, an arbitrary shape provided by you, the shape of object(s) identified by an Identify module, or a shape created using a previous Crop module in the pipeline.

not available anymore in version 3.0 and up?

Fills the holes in all the ROI of the active sequence (also works in Protocols).

Import Matlab *.mat files as sequences in Icy.

OverlayOutlines is a module from CellProfiler to place outlines of objects over a desired image.