comet assay analysis

This workflow will batch process a directory of images: - comets should be horizontally oriented, tails to the right (additional pre-step if needed top reach this requirement: Rotate images, Using ImageJ/Transform Image or TransformJ plugin for exemple) - then included in the plugin: ->Uneven background correction -> automatiic detection of comet shapes with outliers detection -> automatic detection of head of comets (brightest region or profile analysis) -> statistics aboit tails, heads and Olive moments - Manual correction is also available Can be run from Micro-Manager for live analysis See [bib]2621[/bib] for associated paper.