Performing a Fluorescence Recovery after Photobleaching (FRAP) experiment

Type
Workflow
Author
Chan-Ying Zheng
Ronald S. Petralia
Ya-Xian Wang
Bechara Kachar
Requires
ImageJ
Excel
Supported image dimension
2D
time-series
Interaction Level
Automated
License/Openness
I do not know
Description

The article describes how a FRAP experiment can be conducted and subsequently analyzed. This includes steps in ImageJ and subsequent normalization of the intensity data.

This is a qualitative analysis, and curve fitting is done using Excel. 

Requires "Template matching and Slice alignment plugin"

has topic
Confocal fluorescence microscopy
has biological terms
Hippocampal Neurons
Additional keywords
Fluorescence Recovery After Photobleaching
Entry Curator
Niko Kladt
Post date
12/09/2014 - 12:32
Last modified
05/07/2018 - 18:46
Download Page
Jove video tutorial
Reference Publication Link
Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Protei…
DOI link
http://doi.org/10.3791/2568