Contents

This session covers the basics of the R programming language in the context of a bioimage analysis workflow. It aims at getting started with the analysis of data extracted from one or several images, here tracking data. Tracking data were extracted using the Fiji plugin TrackMate and are available in /datasets/fly_embryo/.

Make your first experience with TensorFlow-Keras

Part 1 : https://canal-ua.univ-angers.fr/avc/courseaccess?id=4797

Part 2 : https://canal-ua.univ-angers.fr/avc/courseaccess?id=4796

Part 3 : https://canal-ua.univ-angers.fr/avc/courseaccess?id=4809

FAIRsharing is a database of databases and repositories. The collection gathered by EuroBioImaging contains alist of public database hosting images from biomedical siences. They are classified using a taxonomy for the biological model used (Homo Sapiens, Drosophila,...)

DeepCell is neural network library for single cell analysis, written in Python and built using TensorFlow and Keras.

DeepCell aids in biological analysis by automatically segmenting and classifying cells in optical microscopy images. This framework consumes raw images and provides uniquely annotated files as an output.

The jupyter session in the read docs are broken, but the one from the GitHub are functional (see usage example )

Muscle cross-section with immunofluorescence , to count myofibers

Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic procedure. As a result, embryo viability has been measured primarily using differential interference contrast (DIC).

Raw data of three types of dendritic spines imaged in biphoton (mushroom, tubby, thin spines).

Hippocampal neurons from mouse organotypic slice cultures 

If you use this dataset , please cite

M. U. Ghani, F. Mesadi, S. D. Kanik, A. O. Argunsah, A. Hobbiss, I. Israely, D. Unay, T. Tasdizen, and M. Cetin, "Shape and appearance features based dendritic spine classification," Journal of Neuroscience Methods.

One of the principal challenges in counting or segmenting cells or cell nuclei is dealing with clustered objects such as in tissues. To help assess algorithms' performance in this regard, synthetic 3D image sets of human colon tissue are provided in two diferent levels of quality: high SNR and low SNR. Ground truth is available as well.

One of the principal challenges in counting or segmenting cells or cell nuclei is dealing with clustered objects. To help assess algorithms' performance in this regard, synthetic 3D image sets of HL60 cell line are provided consisting of four subsets with increasing degree of clustering. Each subset is also provided in two diferent levels of quality: high SNR and low SNR. Ground truth is available as well.

Dragonfly is a software platform for the intuitive inspection of multi-scale multi-modality image data. Its user-friendly experience translates into powerful quantitative findings with high-impact visuals, driven by nuanced easy-to-learn controls.

For segmentation: It provides an engine fior machine Learning, Watershed and superpixel methods, support histological data .

It offers a 3D viewer, and python scripting capacities .

It is free for reserach use, but not for commercial usage.

collection of real life science images and videos from australian research labs.

including photographs of plants, microscopy equipments, microscopy images

Web based viewer developped for google for very big data: 

Neuroglancer is a WebGL-based viewer for volumetric data. It is capable of displaying arbitrary (non axis-aligned) cross-sectional views of volumetric data, as well as 3-D meshes and line-segment based models (skeletons). The segmentation has to be done before loading the dataset, it is not done Inside the viewer.

This is not an official Google product.

It has among other the nice feature of beeing able to generate url for sharing a specific view.

Note that the only supported browser for now are 

2,480  benign and 5,429 malignant annotated histophatology dataset of cancer breast tissue from 82 patients

using different magnifying factors (40X, 100X, 200X, and 400X)

The research goal of this paper was to provide unbiased counts of labeled astrocytes and to estimate the area they cover, further to develop tools for defining the orientation of coupling within astrocyte networks under different stimuli.

André Dias, Marco Werner, Kevin R. Ward, Jean-Baptiste Fleuryd and Vladimir A. Baulin

It has become increasingly clear that astrocytes modulate neuronal function not only at the synaptic and single-cell levels, but also at the network level. Astrocytes are strongly connected to each other through gap junctions and coupling through these junctions is dynamic and highly regulated. An emerging concept is that astrocytic functions are specialized and adapted to the functions of the neuronal circuit with which they are associated.

Diagnosing illnesses, developing and comparing treatment methods, and conducting research on the organization of the peripheral nervous system often require the analysis of peripheral nerve images to quantify the number, myelination, and size of axons in a nerve. Current methods that require manually labeling each axon can be extremely time-consuming as a single nerve can contain thousands of axons.

We have developed WormScan, an automated image acquisition system that allows quantitative analysis of each of these four phenotypes on standard NGM plates seeded with E. coli. This system is very easy to implement and has the capacity to be used in high-throughput analysis.

online image data management systemwhich supports authenticated image upload, cloud-based storage, project-based management and viewing of standard and whole slide images. One can use different annotation tools to highlight important objects or areas within images. It is the first basic version and new features such as sharing for easy collaboration with your colleagues or first automated analysis applications based on artificial intelligence will be added soon.

Ikosa Portal: multi user image data management