Component

A Component is an implementation of certain image processing / analysis algorithms.

Each component alone does not solve a Bioimage Analysis problem.

These problems can be addressed by combining such components into workflows.

Moment thresholding (ImageJ)

Component
Description

This method allows to compute a threshold that preserves the moments of an image. In ImageJ/Fiji, you can access it in Image->Ajust->Threshold and choose Moments in the list. In Aphelion, the tool is in Segmentation->Threshold->AphImgMomentThreshold The original paper is 2449

has topic
Bioimage informatics
has function
Image segmentation
Image thresholding
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Extract_Images

Component
Description

A utility macro for the specified use of BioFormats plugin. Takes a folder of proprietary images formats (Zeiss zvi, lsm, czi or Nikon nd2) and extracts them to .tif images

The extracted images are located in a folder defined in the menu. Other options: reset spatial scales, reads ROIs, split channels, add stage position in the name.

<!-- [previous text] This macro allows to batch convert .zvi multichannel time-lapse movies into .tif stacks. There are several options for processing and filtering. In particular, you can register the jitter due to small stage movements during acquisition. For this option to work you need to install Kang Li's Image Stabilizer plugin. -->

note: The old name of the macro was "ZVI Extractor" and the data format was limited to ZVI, but the upgraded version includes more formats.

Requires Bio-Formats plugin

Optical Flow

Component
Description

This protocol computes the optical flow of a 2D+T sequence. The results are displayed with flow arrows painted on top of the original sequence, and also with two additional sequences for the norm of the flow and a color-coded presentation of the flow following the Middlebury convention.

need a thumbnail

LIF Projector

Component
Description

This macro is similar to the LIF_Extractor, but it will output Z-projection of each Z-stack. You can choose the type of projection in addition to the other options. Requires Bio-Formats plugin

has function
Image projection

LIF Extractor

Component
Description

This macro extracts .lei and .lif multichannel Z-stacks into multiple .tif stacks, splitting the channels into different stacks. Several options are possible such as background substraction, various filters, or optional reset of spatial scale. Requires Bio-Formats plugin

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