Automated

A MATLAB package is made available for computing the fractional smoothing spline estimator of a 1D signal, and for generating fBms (fractional Brownian motion).

Icy Morphomath operators: erosion, dilation, opening, closing, top-hat, gradient, distance map, skeleton and watershed.

The smoothing is applied by the way of a bi-exponential filter, itself realized by a pair of one-tap recursions. It is therefore very fast; moreover, its computational cost is truly independent of the amount of smoothing. Meanwhile, the preservation of edges is obtained by a range filter akin to the range filter found in a bilateral filter. More technical details are available here.

The plugin allows one to control the amount of smoothing, the type of range filter, its broadness, and to iterate the filter several times if desired. We illustrate in Figure 2 a possible outcome of this filter. Here, we iterated the BEEPS 10 times with a Gaussian range filter, σ = 10, and the spatial decay λ = 0.1.

ICY Plugin, creates a colocalization scatter plot based on 2 channels. This plugin can take 1 ROI as input, and provides the data to build the bubble excel graph.

Dithering is a type of half tone thresholding where greyscale (or RGB channel) intensity is converted into a local density of binary pixels. This is ideal for rendering images in devices with a binary output such as printers (greyscale) or with a small number of colours (colour dithering). The following methods have been implemented (there are several more):

• Floyd-Steinberg
• Atkinson
• Jarvis-Judice-Ninke
• Stucki
• Bayer_2x2
• Bayer_4x4
• Bayer_8x8
• Clustered_4x4
• Random

Here is a good text explaining various dithering algorithm.