An estimate of the shortest distance of vesicles to synaptic cleft is computed in 3D for serial section TEM. Unfortunately the the authors do not provide an implementation. Method: 1. Bias correction for inhomogene lighting 2. Image registration of TEM sections / stacks 3. Detection of vesicles & synaptic cleft (semi-automatic) 4. Compute distances in 3D
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A Great Guy
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