3D estimation of synaptic vesicle distribution in serial section TEM (ssTEM)

An estimate of the shortest distance of vesicles to synaptic cleft is computed in 3D for serial section TEM. Unfortunately the the authors do not provide an implementation. Method: 1. Bias correction for inhomogene lighting 2. Image registration of TEM sections / stacks 3. Detection of vesicles & synaptic cleft (semi-automatic) 4. Compute distances in 3D