In this chapter you will learn to track cell movements within the monolayer epithelium of a Drosophila embryo.The segmentation of the cells is performed by an ImageJ macro on microscope time lapses (movies) of the embryo presented in the maximum intensity projection (step1). Another macro can optionally be used to discard weak cell–cell junctions that are likely to be segmentation errors(step2). Thet racking is then performed in Matlab from this binary stack (step3). Finally, you will learn how to visualize the results of the cell tracking (step4): the cell area evolution and the cell tracks.