Interaction Level
Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2) has a different gene knocked down by RNAi. The sample preparation is described in more detail by [Carpenter et al. (Genome Biology, 2006)](…) There are 10 fields of view of each sample, for a total of 50 fields of view. The images were acquired on a Zeiss Axiovert 200M microscope. The images provided here are a single channel, DNA. The image size is 512 x 512 pixels. The images are provided as 8-bit TIFF files. This image set belongs to the Broad Bioimage Benchmark Collection (BBBC). The BBBC resource is described in the following publication: Ljosa V, Sokolnicki KL, Carpenter AE (2012). Anno tated high-throughput microscopy image sets for validation. Nature Methods 9(7):637 / doi. PMID: 22743765 PMCID: PMC3627348. Available at [](
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