3D

Wavelet-based method to merge a stack of micrographs taken at different focal positions (aligned along the optical axis) into a single, entirely focused composite image.

This plugin allows the creation of custom animations for 3D viewing. It will generate a new sequence that can be edited in Icy, and saved.

The animation is based on key framing, as in most of 3D rendering software projects.

Layer for the 3D Viewer, allowing the user to move the camera in a more intuitive way.

Outline The incessant development of improved microscopy imaging techniques, as well as the advent of highly selective fluorescent dyes has made possible the precise identification of tagged molecules in almost any biological specimen. Of particular interest are the visualization and the study of living cells, which induce tight constraints on the imaging process. To avoid the alteration of the sample and to achieve a high temporal resolution, low fluorophore concentrations, low-power illumination and short exposure time need to be used in practice. Such restrictions have a tremendous impact on the image quality. This is why we have recently introduced a new method, coined PURE-LET [1,2,3], for efficient, fast, and automatic denoising of multidimensional fluorescence microscopy images.

This plugin provides an extended depth of field algorithm to obtain in focus microscopic images of 3D objects and organisms using different algorithms: Sobel, variance, real and complex wavelets.