2D

Python is a programming language.

Python 2.7.0 was released on July 3rd, 2010.

Python 2.7 is scheduled to be the last major version in the 2.x series before it moves into an extended maintenance period. This release contains many of the features that were first released in Python 3.1.

 A bugfix release, 2.7.16, is currently available. Its use is recommended.

LibTIFF - TIFF Library and Utilities. This software provides support for the Tag Image File Format (TIFF), a widely used format for storing image data. libtiff is a library, for reading and writing TIFF, a small collection of tools for doing simple manipulations of TIFF images.

Matplotlib is a comprehensive library for creating static, animated, and interactive visualizations in Python.

Manual tracking using Trackmate plugin (comes with FIji, so no installation required if you are using Fiji). 

This is the official website of the GNU Image Manipulation Program (GIMP).

GIMP is a cross-platform image editor available for GNU/Linux, OS X, Windows and more operating systems. It is free software, you can change its source code and distribute your changes.

Whether you are a graphic designer, photographer, illustrator, or scientist, GIMP provides you with sophisticated tools to get your job done. You can further enhance your productivity with GIMP thanks to many customization options and 3rd party plugins.

CLI

example

gimp -i -b '(simple-unsharp-mask "foo.png" 5.0 0.5 0)' -b '(gimp-quit 0)'

More details, see here: GIMP Batch Mode