Type
Author
Laurent Gelman
Jens Rietdorf
Requires
Execution Platform
Programming Language
Supported image dimension
Interaction Level
License/Openness
License
Freely available upon request to the author
Description

The macro generates orthogonal projections from bead images along the lateral and axial dimensions which are displayed using a customized look-up-table to color code intensities. A Gaussian curve is fit to the intensity profile of a fluorescent bead image and full-with-at-half-maximum (FWHM) values are extracted, and listed next to theoretical values for comparison. 

Additional keywords