Multiview Reconstruction

Description

The Multiview Reconstruction software package enables users to register, fuse, deconvolve and view multiview microscopy images. The software is designed for lightsheet fluorescence microscopy (LSFM), but is applicable to any form of three or higher dimensional imaging modalities like confocal timeseries or multicolor stacks. 

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BigStitcher

Description

The BigStitcher is a software package that allows simple and efficient alignment of multi-tile and multi-angle image datasets, for example acquired by lightsheet, widefield or confocal microscopes. The software supports images of almost arbitrary size ranging from very small images up to volumes in the range of many terabytes, which are for example produced when acquiring cleared tissue samples with lightsheet microscopy.

TransformJ

Description

A Java Package for Geometrical Image Transformation, works up to 5D.

  • Affine
  • Crop
  • Embed
  • Matrix
  • Mirror
  • Rotate
  • Scale
  • Translate
  • Turn

3D affine transformation based on paired points

Description

Using a text file containing 3D point coordinates as reference pairs, 3D image stack is transformed.

Interactive Affine

Description

quote:

This plugin allows to apply a free affine transformation to a 2D image in an interactive way.

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Affine transformation software

Description

This C routine is based on the following two papers:

  • M. Unser, A. Aldroubi and M. Eden, "B-Spline Signal Processing: Part I--Theory," IEEE Transactions on Signal Processing, vol. 41, no. 2, pp. 821-832, February 1993.
  • M. Unser, A. Aldroubi and M. Eden, "B-Spline Signal Processing: Part II--Efficient Design and Applications," IEEE Transactions on Signal Processing, vol. 41, no. 2, pp. 834-848, February 1993.

It implements the resampling of an image/volume under an affine transformation. The continuous model is based on splines of degree 0 (nearest neighbours), degree 1 (linear interpolation), degree 2 (quadratic), 3 (cubic), 4 (quartic), 5 (quintic), 6 and 7. By convention, the affine transformation is given by an homogenous matrix; the operation performed is output(A x) = input(x). In other words, a matrix given by

A = { {2,0,0,0}, {0,2,0,0}, {0,0,2,0}, {0,0,0,1} }

will result in a magnification by a factor 2 in linear dimensions. In case the desired operation would be output(x) = input(A x), it should be easy to modify the code (mainly: remove the call to invertTrsf() and assign invTRsf = trsf). The origin relative to which the transformation is performed is given with respect to the center of the top-upper-left voxel; the coordinate system is right-handed. Output values in need of extrapolation are set to the value background.

The input volume (the volume to transform) is given by inPtr, a pointer to an array of float values in raster order. More precisely, the values are ordered such that the x values are incremented first, then the y values, finally the z values. The size of the volume is given by nx, ny and nz, respectively. The output volume has necessarily the same size and follows the same organization. Its memory space cannot be shared with the input, and is supposed to be already allocated when the affineTransform() routine is called.

All routines are local, with the exception of the routine to call, named affineTransform(), and the routine errorReport(). The latter is not included in this distribution; its purpose is to display an error message given by a C-string. Else, the code is self-contained (provided a standard ANSI-C environment is available). It consists of only two files: affine.h and affine.c.

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